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Image Search Results
Journal: Oncotarget
Article Title: Combining molecular and immunohistochemical analyses of key drivers in primary melanomas: interplay between germline and somatic variations
doi: 10.18632/oncotarget.23204
Figure Lengend Snippet: N.a.: not amplified. Wild-type: for p16 and NF1 expression, white cell means that the expression of the protein is maintained; for all the genes analysed by molecular methods white cell means wild-type sequence. For MC1R score, wild type corresponds to the 0 score.
Article Snippet: The following antibodies were used: BRAF V600E mutation-specific antibody (Springer-Bio, clone VE1, 1:50 dilution, standard heat-based antigen retrieval was performed for 30 minutes), the NRAS antibody (LifeSpan BioSciences Inc, clone SP174, 1:50 dilution, standard heat-based antigen retrieval was performed for 60 minutes) [ ], specific for Q61R mutation,
Techniques: Amplification, Expressing, Sequencing
Journal: Oncotarget
Article Title: Combining molecular and immunohistochemical analyses of key drivers in primary melanomas: interplay between germline and somatic variations
doi: 10.18632/oncotarget.23204
Figure Lengend Snippet: Representative IHC results for BRAF V600E ( A : positive, B : negative), NRAS Q61R ( C : positive), NF1 ( D : positive) and p16 ( E : positive and F : negative) protein expression. Magnification ×40.
Article Snippet: The following antibodies were used: BRAF V600E mutation-specific antibody (Springer-Bio, clone VE1, 1:50 dilution, standard heat-based antigen retrieval was performed for 30 minutes), the NRAS antibody (LifeSpan BioSciences Inc, clone SP174, 1:50 dilution, standard heat-based antigen retrieval was performed for 60 minutes) [ ], specific for Q61R mutation,
Techniques: Expressing
Journal: Oncotarget
Article Title: Combining molecular and immunohistochemical analyses of key drivers in primary melanomas: interplay between germline and somatic variations
doi: 10.18632/oncotarget.23204
Figure Lengend Snippet: N.a.: not amplified. Wild-type: for p16 and NF1 expression, white cell means that the expression of the protein is maintained; for all the genes analysed by molecular methods white cell means wild-type sequence. For MC1R score, wild type corresponds to the 0 score.
Article Snippet: The following antibodies were used: BRAF V600E mutation-specific antibody (Springer-Bio, clone VE1, 1:50 dilution, standard heat-based antigen retrieval was performed for 30 minutes), the NRAS antibody (LifeSpan BioSciences Inc, clone SP174, 1:50 dilution, standard heat-based antigen retrieval was performed for 60 minutes) [ ], specific for Q61R mutation, NF1 antibody (Santa Cruz Biotechnology Inc., clone sc-20982, 1:20 dilution, standard heat-based antigen retrieval was performed for 60 minutes) and
Techniques: Amplification, Expressing, Sequencing
Journal: Oncotarget
Article Title: Combining molecular and immunohistochemical analyses of key drivers in primary melanomas: interplay between germline and somatic variations
doi: 10.18632/oncotarget.23204
Figure Lengend Snippet: Representative IHC results for BRAF V600E ( A : positive, B : negative), NRAS Q61R ( C : positive), NF1 ( D : positive) and p16 ( E : positive and F : negative) protein expression. Magnification ×40.
Article Snippet: The following antibodies were used: BRAF V600E mutation-specific antibody (Springer-Bio, clone VE1, 1:50 dilution, standard heat-based antigen retrieval was performed for 30 minutes), the NRAS antibody (LifeSpan BioSciences Inc, clone SP174, 1:50 dilution, standard heat-based antigen retrieval was performed for 60 minutes) [ ], specific for Q61R mutation, NF1 antibody (Santa Cruz Biotechnology Inc., clone sc-20982, 1:20 dilution, standard heat-based antigen retrieval was performed for 60 minutes) and
Techniques: Expressing
Journal: Oncotarget
Article Title: Combining molecular and immunohistochemical analyses of key drivers in primary melanomas: interplay between germline and somatic variations
doi: 10.18632/oncotarget.23204
Figure Lengend Snippet: Association between genes and relative statistics
Article Snippet: The following antibodies were used: BRAF V600E mutation-specific antibody (Springer-Bio, clone VE1, 1:50 dilution, standard heat-based antigen retrieval was performed for 30 minutes), the NRAS antibody (LifeSpan BioSciences Inc, clone SP174, 1:50 dilution, standard heat-based antigen retrieval was performed for 60 minutes) [ ], specific for Q61R mutation, NF1 antibody (Santa Cruz Biotechnology Inc., clone sc-20982, 1:20 dilution, standard heat-based antigen retrieval was performed for 60 minutes) and
Techniques:
Journal: Oncotarget
Article Title: Combining molecular and immunohistochemical analyses of key drivers in primary melanomas: interplay between germline and somatic variations
doi: 10.18632/oncotarget.23204
Figure Lengend Snippet: Electropherograms showing the TERT promoter –146C>T somatic mutation ( A ) and the –245T>C polymorphism ( B ); CDKN2A p.G101W germline mutation ( C ) and of MC1R p.V60L germline variant ( D ). Hematoxylin and eosin ( E ), IHC positive staining for BRAF V600E ( F ) and IHC showing loss of expression of p16 protein ( G ). (Magnification ×40). The variant sequence is indicated by an arrow.
Article Snippet: The following antibodies were used: BRAF V600E mutation-specific antibody (Springer-Bio, clone VE1, 1:50 dilution, standard heat-based antigen retrieval was performed for 30 minutes), the NRAS antibody (LifeSpan BioSciences Inc, clone SP174, 1:50 dilution, standard heat-based antigen retrieval was performed for 60 minutes) [ ], specific for Q61R mutation, NF1 antibody (Santa Cruz Biotechnology Inc., clone sc-20982, 1:20 dilution, standard heat-based antigen retrieval was performed for 60 minutes) and
Techniques: Mutagenesis, Variant Assay, Staining, Expressing, Sequencing